Peptide Reconstitution Calculator

What does this calculator work out?

Four figures, every one from the same division. The concentration, in milligrams per millilitre, the number describing the solution. The volume holding whatever quantity you type into the third box. What that volume comes to in units on a U-100 syringe barrel, since that is the scale printed on the syringes most people keep to hand. And the micrograms per unit, the figure worth noting before anything is drawn up, as it is the smallest step the barrel can resolve.

Alter any of the three inputs and all four outputs shift together. Nothing leaves the page: the arithmetic happens right here.

What is reconstitution?

A peptide vial turns up as lyophilised powder. Lyophilisation is freeze drying done under vacuum, and it leaves a dry cake at the base of the vial. Held that way the material stays stable for a long stretch, which is why it ships in that state and why the vial can sit on a shelf unrefrigerated until it is opened.

Reconstitution is the step turning that cake back into a liquid. Water enters through the stopper, the cake dissolves, and the vial then holds a solution of a known strength. The amount of peptide was untouched by the water going in. A 10mg vial holds 10mg before and after, whether 1ml or 5ml was added. What shifted is how that 10mg is spread across a volume, and that is the one thing the water governs.

How is the concentration worked out?

Milligrams in the vial, divided by millilitres of water added. That is all there is to it.

Running it the opposite way is the second job the calculator does. To find the volume holding a given quantity, divide that quantity by the concentration. Take the second row above: 0.5mg at 5 mg/ml is 0.5 divided by 5, which comes to 0.1ml.

A smaller volume of water yields a stronger solution in less total liquid. A larger volume yields a weaker solution across more liquid, and weaker solutions are measured out in bigger, easier volumes on a syringe barrel. Neither alters what the vial contains.

What does a unit on a U-100 syringe mean?

U-100 is a scale, not a size. The barrel is graduated so that 100 units is 1ml, making one unit 0.01ml. A 1ml U-100 syringe reads to 100, a 0.5ml to 50, a 0.3ml to 30, and the gap between marks is the same on all three. The smaller barrels are just shorter and easier to read.

The calculator prints micrograms per unit because a unit is the finest division the barrel offers. At 10 mg/ml one unit holds 100mcg; at 2 mg/ml that same unit holds 20mcg. The weaker the solution, the finer the resolution, which is why the volume of water deserves a deliberate choice rather than a habitual one.

Which water goes into the vial?

Three liquids come up in this setting and they are not interchangeable.

The volume is the same sum whichever of the three goes in. What differs is how long the resulting solution stays usable after the first entry.

Every vial size we stock, at a glance

Drawn from the live catalogue, so it shows the sizes genuinely on the shelf and nothing more. Scan down to the strength on your label, then across to the volume you mean to add.

Figures are mg/ml. A blank would signal a size we do not sell; every row above is a size you can order.

How is the vial handled?

1. Let it reach room temperature. A vial pulled straight from a fridge or freezer goes in cold, and cold powder takes up water more slowly.
2. Clean both stoppers. The peptide vial and the water vial, each with a fresh alcohol swab, every time either is entered.
3. Draw the volume you settled on from the water vial. That figure is the one the calculator gave you.
4. Run it down the glass. Tilt the needle so the water tracks down the inside wall and gathers under the cake rather than landing on top.
5. Swirl, do not shake. Rolling the vial between your fingers does it. Foam on the surface is denatured protein, visible proof of damage.
6. Give it time. Most cakes clear inside a few minutes. A finished solution is clear and free of particles.
7. Label it. Noting the date and the concentration on the vial is what keeps the figure to hand a fortnight on, once the calculator is shut.

A solution that stays cloudy, shows visible strands, or leaves matter on the bottom after a long wait has not fully dissolved. That describes what is in front of you, not a verdict on the batch, and the batch number on the label is what a certificate of analysis is matched against.

How long does a reconstituted vial keep?

Sealed as powder, a lyophilised peptide is the hard-wearing form: years in a freezer near minus 20 degrees, months refrigerated at 2 to 8 degrees, and weeks at room temperature so long as it stays dry and out of light. Once water is in, the clock runs differently. A solution made with bacteriostatic water is usually worked to around four weeks refrigerated, upright and out of light. Sterile water and water for injection carry no preservative, so a vial made up with either is treated as single entry.

A solution has no place in a freezer. Ice crystals form through the liquid and shear the peptide as they grow, and a vial frozen and thawed over and over loses more each cycle. The purity figure on the label records the material as it left testing, not as it stands after a month in a door shelf.

What if the format is already in solution?

Not everything here is a powder, and where it is not, none of the above holds.

Which compounds does this apply to?

Every compound below ships as powder, and each carries this same table in its own strengths on its own page, next to its price and its rate per milligram.

• 5-Amino-1MQ 50mg
• AOD-9604 2mg, 5mg, 10mg
• ARA-290 10mg
• BPC-157 10mg
• BPC-157 + TB-500 10mg
• Cagrilintide 5mg, 10mg
• CJC-1295 DAC 2mg
• DSIP 5mg
• GHK-Cu 50mg, 100mg
• GLOW 50mg, 70mg
• Ipamorelin 5mg, 10mg
• Kisspeptin-10 5mg, 10mg
• KLOW 80mg
• KPV 10mg
• Methylene Blue 10mg
• MOTS-c 5mg, 10mg
• NAD+ 500mg
• PT-141 10mg
• Retatrutide 5mg, 10mg, 20mg, 30mg, 40mg
• Semaglutide 2mg, 5mg, 10mg
• Semax 5mg
• SLU-PP-332 10mg, 50mg
• SS-31 10mg, 50mg
• TB-500 10mg
• Tesamorelin 10mg
• Thymosin Alpha-1 5mg, 10mg
• Tirzepatide 5mg, 10mg, 20mg, 30mg, 40mg, 60mg
• VIP 10mg

Peptide Reconstitution Calculator questions

How much bacteriostatic water do I add to a 10mg vial?

Whatever volume lands you on the concentration you mean to work in, since the vial holds 10mg regardless. 1ml gives 10 mg/ml, 2ml gives 5 mg/ml, 3ml gives 3.33 mg/ml and 5ml gives 2 mg/ml. Enter 10 and your volume into the calculator above and it prints the figure alongside what it reads as on a U-100 syringe.

How do I convert mg to units on an insulin syringe?

A U-100 syringe is graduated 100 units to 1ml, so one unit is 0.01ml. Divide the quantity in milligrams by the concentration in mg/ml for millilitres, then multiply by 100. At 5 mg/ml, 0.5mg is 0.1ml, which is 10 units.

Does adding more water mean less peptide?

No. The vial holds what the label states whatever volume you add. More water spreads the same quantity across more liquid, which drops the concentration and leaves each unit on the barrel holding less.

Can I use sterile water instead of bacteriostatic water?

The arithmetic is the same. The difference lies in the 0.9% benzyl alcohol in bacteriostatic water, which curbs microbial growth and is what lets a vial be entered more than once. Sterile water and water for injection carry no preservative.

How long does a reconstituted vial last?

Made up with bacteriostatic water and held refrigerated, upright and out of light, around four weeks is the figure usually worked to. With sterile water or water for injection there is no preservative, so it is treated as single entry.

Can I freeze a vial after mixing it?

A sealed powder vial freezes well, and that is where long holding belongs. A solution does not. Ice crystals harm the peptide as they form and repeated freeze and thaw cycles add to it.

Why is my solution cloudy?

A cake that has not fully dissolved leaves the liquid hazy or stranded, and more time at room temperature with a gentle swirl is what clears it. Foam on the surface is a separate matter: it comes from shaking, and it is protein damage you can see.

Does the calculator tell me a dose?

No. It converts between milligrams, millilitres and syringe units, which is arithmetic about a solution. Every item sold here is an unlicensed research compound for laboratory research use only, and this site puts out no dosage or protocol guidance.

Do pens need reconstituting?

No. A pen turns up filled, its concentration already fixed, so nothing is added to it. The same goes for the nasal sprays, and capsules are a finished solid.

Where do I find the figures for one specific compound?

On that compound's own page. Each one prints this table in its own strengths under a reconstitution heading, and the full list of compounds that ship as powder is linked above.